PtiCYP85A3, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.
Identifieur interne : 000607 ( Main/Exploration ); précédent : 000606; suivant : 000608PtiCYP85A3, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.
Auteurs : Yanli Jin [République populaire de Chine] ; Chunyan Yu [République populaire de Chine] ; Chunmei Jiang [République populaire de Chine] ; Xiaotong Guo [République populaire de Chine] ; Bei Li [République populaire de Chine] ; Cuiting Wang [République populaire de Chine] ; Fanjing Kong [République populaire de Chine] ; Hongxia Zhang [République populaire de Chine] ; Haihai Wang [République populaire de Chine]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
In angiosperm trees, the gelatinous layer (G-layer) takes a great part of the fiber cell wall in the tension wood (TW). However, the mechanism underlying G-layer formation in poplar is largely unknown. In this work, we demonstrate that G-layer formation in poplar TW cells is regulated by brassinosteroid (BR) and its signaling. PtiCYP85A3, a key BR biosynthesis gene, was predominantly expressed in the xylem of TW, accompanied with a relatively higher castasterone (CS) accumulation, than in the xylem of opposite wood (OW). A wider expression zone of BZR1, a key transcriptional factor in BR singling pathway, was also observed in G-fiber cells on TW side than in wood fiber cells on the OW side, as indicated by immunohistochemistry assays. Transgenic poplar plants overexpressing PtiCYP85A3 produced thicker G-layer with higher cellulose proportion, and accumulated more BZR1 protein in the xylem of TW than did the wild type (WT) plants. Expression of most TW-associated CesAs, which were induced by 2, 4-epibrassinolide, an active BR, and inhibited by brassinazole, a BR biosynthesis inhibitor, were also up-regulated in the xylem of TW in transgenic plants compared to that in WT plants. Further studies with dual-luciferase assays demonstrated that the promoters of PtiCesAs were activated by PtiMYB128, a TW specific transcription factor, which was then regulated by BZR1. All these results indicate that BR plays a crucial role in the G-layer formation of TW fiber cells by regulating the expression of BZR1, PtiMYB128, and PtiCesAs in poplar.
DOI: 10.3389/fpls.2020.00468
PubMed: 32391036
PubMed Central: PMC7193022
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><i>PtiCYP85A3</i>, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.</title><author><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chunyan" sort="Yu, Chunyan" uniqKey="Yu C" first="Chunyan" last="Yu">Chunyan Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Guo, Xiaotong" sort="Guo, Xiaotong" uniqKey="Guo X" first="Xiaotong" last="Guo">Xiaotong Guo</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Cuiting" sort="Wang, Cuiting" uniqKey="Wang C" first="Cuiting" last="Wang">Cuiting Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Fanjing" sort="Kong, Fanjing" uniqKey="Kong F" first="Fanjing" last="Kong">Fanjing Kong</name><affiliation wicri:level="3"><nlm:affiliation>Ministry of Natural Resources Key Laboratory of Saline Lake Resources and Environments, Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Ministry of Natural Resources Key Laboratory of Saline Lake Resources and Environments, Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32391036</idno><idno type="pmid">32391036</idno><idno type="doi">10.3389/fpls.2020.00468</idno><idno type="pmc">PMC7193022</idno><idno type="wicri:Area/Main/Corpus">000310</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000310</idno><idno type="wicri:Area/Main/Curation">000310</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000310</idno><idno type="wicri:Area/Main/Exploration">000310</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en"><i>PtiCYP85A3</i>, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.</title><author><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chunyan" sort="Yu, Chunyan" uniqKey="Yu C" first="Chunyan" last="Yu">Chunyan Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Guo, Xiaotong" sort="Guo, Xiaotong" uniqKey="Guo X" first="Xiaotong" last="Guo">Xiaotong Guo</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Cuiting" sort="Wang, Cuiting" uniqKey="Wang C" first="Cuiting" last="Wang">Cuiting Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Fanjing" sort="Kong, Fanjing" uniqKey="Kong F" first="Fanjing" last="Kong">Fanjing Kong</name><affiliation wicri:level="3"><nlm:affiliation>Ministry of Natural Resources Key Laboratory of Saline Lake Resources and Environments, Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Ministry of Natural Resources Key Laboratory of Saline Lake Resources and Environments, Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In angiosperm trees, the gelatinous layer (G-layer) takes a great part of the fiber cell wall in the tension wood (TW). However, the mechanism underlying G-layer formation in poplar is largely unknown. In this work, we demonstrate that G-layer formation in poplar TW cells is regulated by brassinosteroid (BR) and its signaling. <i>PtiCYP85A3</i>, a key BR biosynthesis gene, was predominantly expressed in the xylem of TW, accompanied with a relatively higher castasterone (CS) accumulation, than in the xylem of opposite wood (OW). A wider expression zone of BZR1, a key transcriptional factor in BR singling pathway, was also observed in G-fiber cells on TW side than in wood fiber cells on the OW side, as indicated by immunohistochemistry assays. Transgenic poplar plants overexpressing <i>PtiCYP85A3</i> produced thicker G-layer with higher cellulose proportion, and accumulated more BZR1 protein in the xylem of TW than did the wild type (WT) plants. Expression of most TW-associated <i>CesAs</i>, which were induced by 2, 4-epibrassinolide, an active BR, and inhibited by brassinazole, a BR biosynthesis inhibitor, were also up-regulated in the xylem of TW in transgenic plants compared to that in WT plants. Further studies with dual-luciferase assays demonstrated that the promoters of <i>PtiCesAs</i> were activated by PtiMYB128, a TW specific transcription factor, which was then regulated by BZR1. All these results indicate that BR plays a crucial role in the G-layer formation of TW fiber cells by regulating the expression of <i>BZR1</i>, <i>PtiMYB128</i>, and <i>PtiCesAs</i> in poplar.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32391036</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle><i>PtiCYP85A3</i>, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.</ArticleTitle><Pagination><MedlinePgn>468</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.00468</ELocationID><Abstract><AbstractText>In angiosperm trees, the gelatinous layer (G-layer) takes a great part of the fiber cell wall in the tension wood (TW). However, the mechanism underlying G-layer formation in poplar is largely unknown. In this work, we demonstrate that G-layer formation in poplar TW cells is regulated by brassinosteroid (BR) and its signaling. <i>PtiCYP85A3</i>, a key BR biosynthesis gene, was predominantly expressed in the xylem of TW, accompanied with a relatively higher castasterone (CS) accumulation, than in the xylem of opposite wood (OW). A wider expression zone of BZR1, a key transcriptional factor in BR singling pathway, was also observed in G-fiber cells on TW side than in wood fiber cells on the OW side, as indicated by immunohistochemistry assays. Transgenic poplar plants overexpressing <i>PtiCYP85A3</i> produced thicker G-layer with higher cellulose proportion, and accumulated more BZR1 protein in the xylem of TW than did the wild type (WT) plants. Expression of most TW-associated <i>CesAs</i>, which were induced by 2, 4-epibrassinolide, an active BR, and inhibited by brassinazole, a BR biosynthesis inhibitor, were also up-regulated in the xylem of TW in transgenic plants compared to that in WT plants. Further studies with dual-luciferase assays demonstrated that the promoters of <i>PtiCesAs</i> were activated by PtiMYB128, a TW specific transcription factor, which was then regulated by BZR1. All these results indicate that BR plays a crucial role in the G-layer formation of TW fiber cells by regulating the expression of <i>BZR1</i>, <i>PtiMYB128</i>, and <i>PtiCesAs</i> in poplar.</AbstractText><CopyrightInformation>Copyright © 2020 Jin, Yu, Jiang, Guo, Li, Wang, Kong, Zhang and Wang.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Yanli</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Chunyan</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Chunmei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Xiaotong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Bei</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Cuiting</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Fanjing</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Ministry of Natural Resources Key Laboratory of Saline Lake Resources and Environments, Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hongxia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Haihai</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">G-layer</Keyword><Keyword MajorTopicYN="N">PtiCYP85A3</Keyword><Keyword MajorTopicYN="N">brassinosteroids</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">tension wood</Keyword><Keyword MajorTopicYN="N">xylem</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32391036</ArticleId><ArticleId IdType="doi">10.3389/fpls.2020.00468</ArticleId><ArticleId IdType="pmc">PMC7193022</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Planta. 2012 Mar;235(3):485-98</Citation><ArticleIdList><ArticleId IdType="pubmed">21947665</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1999 Mar;208(1):1-11</Citation><ArticleIdList><ArticleId IdType="pubmed">10212999</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Nov;169(3):2048-63</Citation><ArticleIdList><ArticleId IdType="pubmed">26378099</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Aug;17(8):2397-412</Citation><ArticleIdList><ArticleId IdType="pubmed">16024588</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2008 Oct;20(10):2763-82</Citation><ArticleIdList><ArticleId IdType="pubmed">18952777</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2017 Oct;15(10):1309-1321</Citation><ArticleIdList><ArticleId IdType="pubmed">28258966</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2002 Sep;31(6):675-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12220260</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5984-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19293381</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Nov;157(3):1452-68</Citation><ArticleIdList><ArticleId IdType="pubmed">21908685</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2013 Aug;33(8):878-86</Citation><ArticleIdList><ArticleId IdType="pubmed">23939552</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jun;126(2):770-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11402205</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2015 Mar 10;16:164</Citation><ArticleIdList><ArticleId IdType="pubmed">25886950</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Nov;56(4):531-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18643995</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2017 Nov;246(5):857-878</Citation><ArticleIdList><ArticleId IdType="pubmed">28699115</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 May 6;280(18):17873-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15710611</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2015 Mar;66(5):1291-302</Citation><ArticleIdList><ArticleId IdType="pubmed">25428999</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2001 Sep;42(9):1006-11</Citation><ArticleIdList><ArticleId IdType="pubmed">11577196</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2001 Nov;47(4):491-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11669574</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2008 May;227(6):1409-14</Citation><ArticleIdList><ArticleId IdType="pubmed">18320214</ArticleId></ArticleIdList></Reference><Reference><Citation>J Vis Exp. 2010 Mar 11;(37):</Citation><ArticleIdList><ArticleId IdType="pubmed">20224547</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2018 Jul;177(3):1096-1107</Citation><ArticleIdList><ArticleId IdType="pubmed">29760198</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Jun;144(2):1166-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17468227</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1996 Apr 19;85(2):171-82</Citation><ArticleIdList><ArticleId IdType="pubmed">8612270</ArticleId></ArticleIdList></Reference><Reference><Citation>Development. 2004 Nov;131(21):5341-51</Citation><ArticleIdList><ArticleId IdType="pubmed">15486337</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1994 Apr;60(4):1383-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16349245</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1761-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9990098</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 May;34(3):339-49</Citation><ArticleIdList><ArticleId IdType="pubmed">12713540</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2008 Oct 3;374(4):614-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18656444</ArticleId></ArticleIdList></Reference><Reference><Citation>C R Biol. 2010 Apr;333(4):325-34</Citation><ArticleIdList><ArticleId IdType="pubmed">20371107</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Jan;140(1):150-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16361521</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Cell Dev Biol. 2010;26:605-37</Citation><ArticleIdList><ArticleId IdType="pubmed">20590454</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 May;135(1):212-20</Citation><ArticleIdList><ArticleId IdType="pubmed">15122024</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2009 May;14(5):248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">19375973</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Jun;46(5):747-57</Citation><ArticleIdList><ArticleId IdType="pubmed">16709191</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2011 Aug;67(3):499-512</Citation><ArticleIdList><ArticleId IdType="pubmed">21649762</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15566-71</Citation><ArticleIdList><ArticleId IdType="pubmed">17878302</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Methods. 2005 Dec 18;1:13</Citation><ArticleIdList><ArticleId IdType="pubmed">16359558</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15572-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17878303</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1996 Apr 19;272(5260):398-401</Citation><ArticleIdList><ArticleId IdType="pubmed">8602526</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(1):e30798</Citation><ArticleIdList><ArticleId IdType="pubmed">22292043</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2018 May;218(3):999-1014</Citation><ArticleIdList><ArticleId IdType="pubmed">29528503</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2015 Jan;86:91-99</Citation><ArticleIdList><ArticleId IdType="pubmed">25438141</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Jan;155(1):562-70</Citation><ArticleIdList><ArticleId IdType="pubmed">21068364</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Apr;143(4):1894-904</Citation><ArticleIdList><ArticleId IdType="pubmed">17322341</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2013 Nov;25(11):4324-41</Citation><ArticleIdList><ArticleId IdType="pubmed">24280390</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Jul 21;7(1):6182</Citation><ArticleIdList><ArticleId IdType="pubmed">28733593</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2005 Apr;42(1):23-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15773851</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1998 Feb;10(2):231-43</Citation><ArticleIdList><ArticleId IdType="pubmed">9490746</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2002 Nov;32(4):495-508</Citation><ArticleIdList><ArticleId IdType="pubmed">12445121</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2002 Dec;216(2):280-92</Citation><ArticleIdList><ArticleId IdType="pubmed">12447542</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2011 Aug;62(13):4495-506</Citation><ArticleIdList><ArticleId IdType="pubmed">21617247</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1450-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12538856</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2015 Jul;66(14):4119-31</Citation><ArticleIdList><ArticleId IdType="pubmed">25750422</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2011 Nov;234(5):959-77</Citation><ArticleIdList><ArticleId IdType="pubmed">21688015</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1996 Jun;8(6):959-69</Citation><ArticleIdList><ArticleId IdType="pubmed">8672892</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2002 Nov;130(3):1152-61</Citation><ArticleIdList><ArticleId IdType="pubmed">12427982</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2001 Sep;47(1-2):239-74</Citation><ArticleIdList><ArticleId IdType="pubmed">11554475</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Pékin</li><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name></noRegion><name sortKey="Guo, Xiaotong" sort="Guo, Xiaotong" uniqKey="Guo X" first="Xiaotong" last="Guo">Xiaotong Guo</name><name sortKey="Guo, Xiaotong" sort="Guo, Xiaotong" uniqKey="Guo X" first="Xiaotong" last="Guo">Xiaotong Guo</name><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><name sortKey="Kong, Fanjing" sort="Kong, Fanjing" uniqKey="Kong F" first="Fanjing" last="Kong">Fanjing Kong</name><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><name sortKey="Wang, Cuiting" sort="Wang, Cuiting" uniqKey="Wang C" first="Cuiting" last="Wang">Cuiting Wang</name><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name><name sortKey="Yu, Chunyan" sort="Yu, Chunyan" uniqKey="Yu C" first="Chunyan" last="Yu">Chunyan Yu</name><name sortKey="Yu, Chunyan" sort="Yu, Chunyan" uniqKey="Yu C" first="Chunyan" last="Yu">Chunyan Yu</name><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000607 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000607 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32391036
|texte= PtiCYP85A3, a BR C-6 Oxidase Gene, Plays a Critical Role in Brassinosteroid-Mediated Tension Wood Formation in Poplar.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32391036" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |